In a televised video on Iranian state television on Thursday, Khamenei cautioned against any future US assaults, marking his return to the spotlight since June 19.
The 86-year-old looked and sounded more tired than he did only a week ago, speaking in a hoarse voice and occasionally stumbling over his words.
A monitoring group, which has been documenting casualties in Iran, reported that out of the total 735 people killed, 417 were civilians and 318 were members of security forces.
At least 28 people were killed in Israel and more than 1000 wounded, according to officials there.
Throughout the 12-day conflict, Iran launched over 550 missiles towards Israel, achieving a 90% interception rate, as per the latest data disclosed by Israeli authorities on Thursday. Israel, on the other hand, targeted more than 720 Iranian military facilities and eight nuclear sites, as confirmed by Israel.
Trump has also asserted that American and Iranian officials will talk next week, giving rise to cautious hope for longer-term peace.
Iran has not acknowledged any such talks would take place, though US Middle East envoy Steve Witkoff has said there has been direct and indirect communication between the countries. A sixth round of US-Iran negotiations was scheduled for earlier this month in Oman but was canceled after Israel attacked Iran on June 13.
Iran has insisted that it will not give up its nuclear program. In a vote underscoring the tough path ahead, its parliament agreed Wednesday to fast-track a proposal that would effectively stop the country’s cooperation with the IAEA, which has monitored the program for years.